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. 2021 Jul 13;153(8):e202112873. doi: 10.1085/jgp.202112873

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© 2021 Lopez-Redondo et al.

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Figure S8. — Steps in structure determination of the YiiP/Fab complex treated with both EDTA and TPEN from the SP4 dataset. (A and B) Representative micrographs from two different imaging sessions. (C) Heterogeneous refinement job in cryoSPARC revealed the presence of two distinct conformations, which were individually used for high-resolution refinement. (D) Nonuniform refinement job in cryoSPARC colored according to local resolution, which ranged from 3.3 to 7.0 Å. Fourier shell correlation (FSC) plots indicate an overall resolution of 3.5 and 4.7 Å for the symmetric conformation and the bent conformation, respectively. (E) Three principal components derived from 3-D variability analysis of the combined particles from both classes.