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. 2021 Jun 30;10:e64507. doi: 10.7554/eLife.64507

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© 2021, Pérez-Vargas et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 2. — (A) HDV RNA titers (GE/μl) in crude supernatants from producer cells co-transfected with pSVL-D3 and with i) empty plasmid (noGP), ii) wild type HBV LMS GPs (Wt), iii) HBV S only (S), or iv) HBV LMS mutant T303C (Cys mutant), and in 100-fold concentrated virus samples subjected to ultracentrifugation through 30% sucrose cushion (UC). (B) Western blot analysis of 100-fold concentrated particles (1E7 GE) from ultrafiltrated crude supernatants or pellets after ultracentrifugation with anti-HBsAg antibody (Murex) and rabbit polyclonal serum against HDAg.