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. 2021 Jul 1;12:651279. doi: 10.3389/fmicb.2021.651279

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Copyright © 2021 Hossain, Pérez-López, Todd, Wei and Bonham-Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of ^ΔspPbPE13GFP to plasma membrane (PM) lipid rafts in N. benthamiana leaf cells. PM localization of ^ΔspPbPE13GFP, together with punctate structures at the cell peri2phery. Co-localization images of ^ΔspPbPE13GFP and a known PM-marker, mCherry-tagged Arabidopsis aquaporin AtPIP2A (CD3-1007). ^ΔspPbPE13GFP did not colocalize with perinuclear ER (white arrow). Co-localization of ^ΔspPbPE13GFP with Arabidopsis REMORIN1.3mRFP in PM lipid rafts. Representative fluorescence intensity plots of co-localization of ^ΔspPbPE13GFP with PM or ER or Remorin markers are shown along the light blue line on image overlays. Scale bars = 10 μm.