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. 2021 Jul 8;2021:7806370. doi: 10.1155/2021/7806370

Figure 3.

Figure 3

Activation of Gi pathway in VMH astrocytes decreases anxiety-like behavior and prevents bone loss. (a) Schematic of experimental procedure for mice with chronic stress and chemogenetic inhibition. (b) Representative image showing GFAP and hM4Di virus expression in VMH of S100B-Cre mouse (green, GFAP; red, hM4Di-mCherry; blue, DAPI; scale bars, 60 μm). (c) Representative heat maps of hM4Di and mCherry mice in different positions in OFT. (d) Representative heat maps of hM4Di and mCherry mice in different positions in EPM. (e) Quantification of entries into and time spent in central area in hM4Di and mCherry groups; values represent mean ± SD (n = 10 for hM4Di and n = 8 for mCherry group; ∗p < 0.05; ∗∗p < 0.01; unpaired t-test). (f) Quantification of entries into and time spent in open arm in hM4Di and mCherry groups; values represent mean ± SD (n = 10 for hM4Di and n = 8 for mCherry group; ∗p < 0.05; unpaired t-test). (g) Representative dual-energy X-ray image showing BMD in hM4Di and mCherry groups. BMD in red box was collected for statistical analysis. (h) Quantification of BMD in hM4Di and mCherry groups; values represent mean ± SD (n = 14 from 7 hM4Di mice and n = 10 from 5 mCherry mice; ∗∗p < 0.01; unpaired t-test). (i) Representative images of H&E staining showing chemogenetic inhibition of VMH astrocytes rescued the decrease in proximal tibia trabeculae caused by chronic stress (scale bar, 100 μm).