Skip to main content
. 2021 Jul 8;2021:7806370. doi: 10.1155/2021/7806370

Figure 4.

Figure 4

Activation of Gq pathway in VMH astrocytes increases anxiety-like behavior and promotes bone loss. (a) Schematic of experimental procedure for mice with chemogenetic activation. (b) Representative image showing GFAP and hM3Dq virus expression in VMH of S100B-Cre mouse (green, GFAP; red, hM3Dq-mCherry; blue, DAPI; scale bars, 60 μm). (c) Representative heat maps of hM3Dq and mCherry mice in different positions in OFT. (d) Representative heat maps of hM3Dq and mCherry mice in different positions in EPM. (e) Quantification of entries into and time spent in central area in hM3Dq and mCherry groups; values represent mean ± SD (n = 10 for hM3Dq and n = 10 for mCherry group; ∗p < 0.05; ∗∗p < 0.01; unpaired t-test). (f) Quantification of entries into and time spent in open arm in hM3Dq and mCherry groups; values represent mean ± SD (n = 10 for hM4Di and n = 10 for mCherry group; ∗p < 0.05; unpaired t-test). (g) Representative dual-energy X-ray image showing BMD of hM3Dq and mCherry groups. BMD in red box was collected for statistical analysis. (h) Quantification of BMD in hM3Dq and mCherry groups; values represent mean ± SD (n = 14 from 7 hM3Dq mice and n = 16 from 8 mCherry mice; ∗∗p < 0.01; unpaired t-test). (i) Representative image of H&E staining showing that chemogenetic activation of VMH astrocytes mimicked the decrease in proximal tibia trabeculae caused by chronic stress (scale bar, 100 μm).