Figure 3.

Total Aβ₄₀ and Aβ₄₂ contents determined by ELISA. Although cerebrocortical Aβ₄₀ contents (A) were similar in the WT, 3xTg-AD, AD+IHT and AD+sham-IHT mice, cerebrocortical Aβ₄₂ (B), and the Aβ₄₂/Aβ₄₀ ratio (C) were greater in the 3xTg-AD than WT mice. Hippocampal Aβ₄₀ content was marginally increased (P = 0.057) in the 3xTg-AD vs. WT mice (D), and hippocampal Aβ₄₂ (E) and the Aβ₄₂/Aβ₄₀ ratio (F) were greater in the 3xTg-AD than WT mice. Both the time factor (P = 0.087) and the treatment factor (P = 0.081) tended to significantly influence the cerebrocortical Aβ₄₂ according to two-factor ANOVA. However, neither these factor altered the hippocampal Aβ₄₂ vs. the pre-intervention 3xTg-AD group. Aβ₄₀ contents or Aβ₄₂/Aβ₄₀ ratios in the cerebral cortex or hippocampus were not affected by the time or treatment factors. Individual data points and group mean ± standard error of the mean are plotted. *P < 0.05, **P < 0.01 for comparisons indicated by horizontal lines.