Table 2.
Known SARS-CoV-2 negative (N = 26) and positive (N = 111) samples tested with the developed assay for validation purposes.
| Sample panel | Mutation determination method | No. of samples | Variant | Fluorescence channel |
||||
|---|---|---|---|---|---|---|---|---|
| FAM | HEX | Texas Red | Cy5 | |||||
| Human | Negative | N/A | 20 | N/A | – | – | – | – |
| Positive (A) | Obtained in September 2020, before the emergence of the targeting mutations | 20 | WT | + | + | – | – | |
| ViroBOAR Spike 1.0 RT-PCR Kit (SARS-CoV-2) & Roche LightCycler 480 II instrument | 4 | WT | + | + | – | – | ||
| 21 | Alpha (B.1.177) | + | – | – | + | |||
| 1 | Beta (B.1.351) | – | – | + | + | |||
| Positive (B) | NGS (Illumina MiSeq) | 16 | WT (B.1.177) | + | + | – | – | |
| 2 | WT (B.1.258) | + | + | – | – | |||
| 29 | Alpha (B.1.1.7) | + | – | – | + | |||
| 12 | Beta (B.1.351) | – | – | + | + | |||
| 1 | Other (B.1.1.318) | – | + | + | – | |||
| Veterinary | Negative | N/A | 3 cats, 3 minks | N/A | – | – | – | – |
| Positive | NGS (Ion Torrent GeneStudio S5) | 2 cats | WT (B.1.1) | + | + | – | – | |
| NGS (Illumina MiSeq) | 3 minks | WT (B.1.1.305) | + | + | – | – | ||
N/A: not applicable; WT: wild type; NGS: next-generation sequencing.