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Optimisation of SARS-CoV-2 S protein yields by alteration of the sequence construct.
| Spike Construct | Gag total amount (mg) | SARS-CoV-2 S total amount (mg | S to Gag ratio (%) | Particle number/mL |
|---|---|---|---|---|
| Native (S) | 23 | 0.16 | 0.7 | 4.37 × 1011 |
| Native-VSV-G (SG) | 19 | 0.5 | 2.63 | 4.37 × 1011 |
| Prefusion (SP) | 32 | 0.23 | 0.72 | 4.37 × 1011 |
| Prefusion-VSV-G (SPG) | 23 | 0.64 | 2.78 | 4.37 × 1011 |
