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. 2021 Jul 1;16(7):1805–1817. doi: 10.1016/j.stemcr.2021.06.002

Figure 1.

Figure 1

Identification of C-Kit-mCherry; Rosa26-lsl-GCaMP5 mESC-derived retinal organoids

(A) Schematic graph showing the gene-targeting strategy used to generate the C-Kit-mCherry; Rosa26-lsl-GCaMP5 mESC line.

(B–E) Images of mEROs differentiation; re-aggregation of dissociated mESC in a V-bottomed 96-well plate (B), optic-vesicle (C), two-walled optic cup-like retinal anlage (D and E).

(F–I) Both of the inner portion (neural retina) and outer wall (pigment epithelium) expressed calcium indicator GCaMP5 (G and I); within an optic cup-like structure, the neural retina strongly expressed RAX, and the pigmented epithelium strongly expressed MITF (F and H) on day 10. Scale bars, 200 μm (D), 100 μm (B, C, and E–G), and 50 μm (H and I).