Skip to main content
. 2021 Jul 1;16(7):1805–1817. doi: 10.1016/j.stemcr.2021.06.002

Figure 2.

Figure 2

Enrichment of C-Kit+/SSEA1− RPCs derived from mEROs

(A) Distribution of C-Kit-mCherry-positive cells at day 11, 15, 20, and 25 neural retina (NR) of mEROs, The intensity of the signal in the NR was confined to the most central region in a temporospatial manner and peaked at day 20.

(B) At day 20 of mEROs, strong C-Kit expression was observed at the basal side of the NR. Lower panels present an apical-basal pattern of photoreceptors and ganglion cells, immunoreactive for CRX and PAX6, respectively.

(C–E) Flow cytometric analysis of C-Kit expression at various developmental stages of mEROs. Density plot pattern of C-Kit-mCherry versus antibody against SSEA1 at differentiation day 15 (C). Percentage of C-Kit-mCherry-positive cells and SSEA1::APC-positive cells among total NR cells from various developmental stages. Error bars, means ± SEM (C-Kit: day 8, 0.35% ± 0.17%; day 11, 5.68% ± 1.48%; day 13, 27.28% ± 3.19%; day 15, 32.84% ± 3.96%; day 18, 40.83% ± 6.69%; day 20, 61.40% ± 8.71%; day 23, 59.45% ± 4.00%; day 27, 44.74% ± 9.83%; day 35, 34.38% ± 6.35%; SSEA1: day 8, 72.03% ± 1.73%; day 11, 25.28% ± 12.30%; day 13, 20.59% ± 1.64%; day 15, 18.94% ± 3.77%; day 18, 13.75% ± 2.21%; day 20, 11.00% ± 2.93%; day 23, 7.61% ± 4.56%; day 27, 8.42% ± 3.21%; day 35, 7.87% ± 1.54%; n = 3 independent experiments/group; each experiment containing 144 EROs) (D). Percentage of C-Kit+/SSEA1− cells among total NR cells at different developmental stages (day 11, 5.68% ± 1.48%; day 15, 32.84% ± 3.96%; day 20, 61.40% ± 8.71%; day 27, 44.74% ± 9.83%; Error bars, means ± SEM; n = 3 independent experiments/group; each experiment containing 144 EROs) (E). Percentages were calculated by using FlowJo v.X.0.7 software. Scale bars, 100 μm (J).