Figure 1.

Efficient generation of eosinophils from hESCs

(A) Experimental schematic for the differentiation of hESCs into eosinophils.

(B) Representative flow plots from four independent experiments showing the generation of EPX⁺ cells during eosinophil induction from E0 to E20.

(C) Percentage of EPX⁺ cell population during the eosinophil induction process from starting H1 cells (n = 4 independent experiments). Data shown as the mean value ± SD.

(D) Representative flow plots from three independent experiments showing staining for the indicated surface marker on H1-derived eosinophils recovered on E20.

(E) Giemsa staining of induced cells recovered on E20. Scale bar, 20 μm.

(F) Electron microscope image of induced cells recovered on E16. Scale bar, 2 μm.

(G) Representative eosinophil yield at E16 from one H1 initiated cell (n = 3 independent experiments). Data shown as mean value ± SD.

See also Figure S1.