Figure 1.

XVIVO medium, but not OXM medium, contains undisclosed molecules

Negative-ion high-resolution LC-MS spectroscopy was used to analyze different compounds in the media.

(A) Total ion chromatogram of the molecules in the XVIVO medium is compared with that of molecules in the OXM medium.

(B) Concentration of glucose in XVIVO and OXM media. n = 3 (XVIVO) and 5 (OXM) independent experiments. Error bars represent ±SD.

(C–E) (C) Comparison of the observed MS spectrum of the main peaks absent in OXM with the simulated spectrum of [M-H]^– adduct of isolariciresinol 9′-O-alpha-L-arabinofuranoside (^∗), (D) that of [M + FA-H] – adduct of methyl glucosinolate (^∗∗), and (E) that of [M-H]^– adduct of dithionous acid (^∗∗∗).