Figure 2.

Morphology throughout macrophage differentiation

(A) Schematic of iPSC-derived macrophage differentiation. Base medium is displayed on the left with main growth factor supplements adjacent.

(B) Average weekly yield (left) and overall average weekly yield (right) of PreMac cells per mL of medium removed from differentiation cultures.

(C) Cumulative yield of PreMac cells over 16 weeks of differentiation (left) and final total yield (right).

(D) PreMac size over the lifespan of the differentiation culture (left) and overall average PreMac size (right).

(E) Methylene blue (1%, w/v) staining to show macrophage morphology.

(F) Macrophage size over multiple weeks of differentiation (left) and overall average macrophage size (right).

(G) Percentage adherent cells cultured in fresh differentiation medium (M-CSF + IL-3) or macrophage medium (M-CSF) after incubation with Accutase normalized to untreated control.

(H) Average of adherent cells over time course shown in (G). (B–H) XVIVO-cultured cells represented in black versus OXM-cultured cells in blue, mean ± SD. (B–C) n = 2 experiments in each of 3 independent donors’ cell lines. (D and F) n = 1 experiment in each of 3 independent donors’ cell lines. (G–H) n is displayed within the bars of (H) and made up from 2 independent donors’ cell lines. (B–F) Significance was calculated by Wilcoxon matched-pairs signed rank t test. (H) Significance was calculated by two-way ANOVA, Tukey’s multiple comparison test. Significance is shown when ^∗p < 0.05, ^∗∗ p < 0.01, ^∗∗∗ p < 0.001.