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. 2021 Jun 24;16(7):1735–1748. doi: 10.1016/j.stemcr.2021.05.018

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Macrophage surface marker phenotype

Surface expression of (A) CD14, (B) CD45, (C) CD163, (D) CD16, (E) CD11b, (F) CD86, (G) HLA-DR, and (H) CD68 as measured by flow cytometry on macrophages. Histograms show fluorescence intensity (x axis) normalized to the mode (y axis) for macrophages cultured in XVIVO (black) or OXM (blue), relative to the isotype control (gray). Dot plots show ratio of the geometric mean fluorescence intensity (MFI) compared with the isotype control. Bars display mean ± SD. n = 4 experiments in each of 3 independent donors’ cell lines. Significance was calculated by Wilcoxon matched-pairs signed rank t test. Significance is shown when ^∗p < 0.05, ^∗∗ p < 0.01, ^∗∗∗ p < 0.001.