Figure 5.

Macrophage activation states between culture media

(A) Cytokine secretion into the supernatant under resting conditions (top), or stimulated with 100 ng/mL LPS + 20 ng/mL IFN-γ (middle) or 50 ng/mL IL-4 (bottom) normalized to a positive control. n = 1 experiment.

(B) TNF-α secretion from unstimulated (black), or 100 ng/mL LPS (red) or 50 ng/mL IL-4 (purple) stimulated cells. Mean ± SD, n = 5 experiments in each of 3 independent donors’ cell lines.

(C) Surface expression of CD45, CD86, or CD206 measured by flow cytometry after stimulation, colored according to (B). Histograms show fluorescence intensity (x axis) normalized to the mode (y axis).

(D) Geometric MFI relative to unstimulated control.

Mean ± SD, n = 3 experiments in each of 2 independent donors’ cell lines. (B–D) Significance was calculated by two-way ANOVA, Sidak’s multiple comparison test. Significance is shown when ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.