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. 2021 Jul 15;8:177. doi: 10.1038/s41597-021-00972-1

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© The Author(s) 2021, corrected publication 2021

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Fig. 1 — Outline of laboratory and bioinformatic workflow. The workflow comprises isolation of (a) mononuclear cells from milk or (b) pbMECs from bulk mammary gland tissue, preparation of single-cell suspensions, library construction, sequencing and (c) the final bioinformatic scRNA-seq workflow. Adapted from Liu et al.³¹.