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. 2021 Jul 14;220(9):e202005193. doi: 10.1083/jcb.202005193

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© 2021 Bomba-Warczak et al.

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Figure S4. — Long-lived subunits of OXPHOS complexes integrate into individual and higher-order complexes. (A) Solubilized cortical mitochondria extracts isolated from ¹⁵N-pulsed mice separated by BN-PAGE to resolve mitochondrial complexes and supercomplexes. Representative Coomassie (Coom.)-stained BN-PAGE gel and Western blot probed with an OXPHOS antibody cocktail. Mitochondrial complexes and supercomplexes are annotated. (B and C) 40 gel slices excised from each gel lane were subjected to in-gel trypsin digestion and LC-MS/MS analysis. FA values were calculated for each protein in a given complex and plotted. C.I, complex I; C.II, complex II; C.III, complex III; C.IV, complex IV; CV_m, complex V monomer; CV_d, complex V dimer; CV_o, complex V oligomer; SC, supercomplex. Data points in B and C, n = 4 mice; mean ± SEM.