Table 1.
Summary of the relative Gi signaling and total expression of BILF1 variants
| BILF1 variant | relative Gi signaling (GαΔ6qi4myr-IP3, 10 ng DNA/well) |
relative expression (ELISA, 50 ng DNA/well) |
|---|---|---|
| wild-type | 100% | 100% |
| K122A | 16.0 ± 4.5 % (**) | 151.3 ± 16.4% (**) |
| ECL2GGG | 63.8 ± 2.8% (**) | 25.2 ± 7.3% (**) |
| ECL2CXCR4a | 63.9 ± 6.4% (**) | 32.1 ± 2.0% (**) |
| ECL2CXCR4b | 58.5 ± 5.7% (**) | 37.3 ± 0.7% (**) |
| ECL2CxCR4ab | 59.5 ± 4.4% (**) | 27.1 ± 4.1% (**) |
| TM6x7-4A | 89.1 ± 8.8% (*) | 111.1 ± 1.4% (*) |
| TM6x7-4A1F | 78.0 ± 5.1% (**) | 108.5 ± 2.7% (ns) |
| A271F | 84.3 ± 13.2% (**) | 66.4 ± 13.5% (**) |
| M240F | 112.6 ± 7.5% (*) | 92.9 ± 9.0% (ns) |
| L241F | 91.5 ± 2.8% (*) | 91.7 ± 9.0% (ns) |
| Y282A | 41.8 ± 4.2% (**) | 129.7 ± 7.1% (**) |
| E121A | 55.1 ± 2.0% (**) | 58.7 ± 3.2% (**) |
| H115A | 58.1 ± 4.9% (**) | 139.4 ± 7.2% (**) |
| L75A | 50.4 ± 10.4% (**) | 97.8 ± 7.5% (ns) |
| NPxxY | 59.1 ± 3.8% (**) | 84.8 ± 7.8 (*) |
| CWxM | 76.8 ± 3.2% (**) | 100.7 ± 4.9% (ns) |
| CWxP | 77.8 ± 3.7% (**) | 98.2 ± 10.6% (ns) |
| SWxP | 92.0 ± 8.6% (ns) | 90.5 ± 3.6% (ns) |
| A125F | 76.8 ± 0.8% (**) | 88.2 ± 3.2% (*) |
| H288A | 98.9 ± 5.7% (ns) | 91.5 ± 7.1% (ns) |
| F286-H288A | 87.1 ± 4.2% (**) | 85.0 ± 9.3% (*) |
| H66A-F286A-H288A | 76.0 ± 2.3% (**) | 79.7 ± 6.1% (**) |
ns: not significant
*:
p < 0.05
**:
p < 0.01