Figure 5.

Increased PAOx metabolism alters the expression of genes related to auxin response, tryptophan metabolism, and phenylpropanoid biosynthesis.

A) The number of genes with significantly increased expression in CYP79A2 overexpression line ox-2 (ox-2-up), ref2 (ref2-up), and ref5 (ref5-up), compared to wild type. B) The number of genes with significantly decreased expression in CYP79A2 overexpression line ox-2 (ox-2-down), ref2 (ref2-down), and ref5 (ref5-down), compared to wild type. C) The expression levels (log₂ fold change) of select genes differentially regulated in ox-2 compared to wild type. D) The expression levels of the KFB genes that are upregulated in ref2, ref5, and ox-2 compared to wild type. E) Relative expression, determined by qRT-PCR, of KFB genes in ox-1 and ox-2 compared to wild type (N=3). 3rd to 6th rosette leaves from two-week-old plants were harvested for RNA extraction. Data represent mean ± SE. The means were compared by one-way ANOVA, and statistically significant differences (P < 0.05) were identified by Tukey’s test and indicated by letters to represent difference among groups.