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. 2021 Jul 15;12:4333. doi: 10.1038/s41467-021-24650-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PACAP38:PAC1R:Gs (PAC1R) dataset collected in part with the Volta phase plate (4032 micrographs) (b), and in part with defocus phase contrast (3617 micrographs) (c). d Taspoglutide:GLP-1R:Gs (GLP-1R-TAS) dataset acquired partially with zero-loss energy filtering (5508 micrographs) (e), and partially without energy filtering (3251 micrographs) (f). g GLP-1:GLP-1R:Gs (GLP-1R-GLP-1) dataset acquired in part with a 100 μm objective lens aperture (3003 micrographs) (h), and in part without an aperture (2736 micrographs) (i). Scale bars 20 nm.