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. 2021 Jul 15;12:4333. doi: 10.1038/s41467-021-24650-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Over the year and a half period shown in the plot, there is a general trend towards better resolution with a significant improvement in August 2019, when the sample supports were switched from holey carbon films to gold foil grids. The size of each dot represents the cryo-EM efficiency quantity (cryoEF, see values in the legend) that estimates the uniformity of the particle orientation distribution. A value of ~0.5 represents an angular distribution that is barely sufficient for producing a usable 3D map and a value of 1 corresponds to an ideal uniform distribution. Published results are annotated and the three datasets analyzed in this study are highlighted in pink.