Table 2.
Data processing details.
| PAC1R | GLP-1R-TAS | GLP-1R-GLP-1 | GLP-1R-GLP-1 | GLP-1R-GLP-1 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| +VPP | −VPP | +ZLF | −ZLF | +OLA | −OLA | Def. > 1 µm |
Def. < 1 µm |
Exp. 65 e/Å2 |
Exp. 40 e/Å2 |
|||
| 212 Å box | 332 Å box | 212 Å box | 332 Å box | |||||||||
| Micrographs | 4032 | 3617 | 5508 | 3251 | 3003 | 2736 | 3013 | 2726 | 5739 | 5739 | ||
| Micrographs after CTF fits (retention) |
3676 (91%) |
3466 (96%) |
4631 (84%) |
2487 (76%) |
2098 (70%) |
2477 (91%) |
2288 (76%) |
2288 (84%) |
4575 (80%) |
4742 (83%) |
||
| Micrographs used | 3466 | 3466 | 2487 | 2487 | 2098 | 2098 | 2288 | 2288 | 4575 | 4742 | ||
| Measured defocus [μm] | 0.4–1.2 | 0.7–1.7 | 0.6–1.8 | 0.6–1.8 | 0.6–1.45 | 0.6–1.45 | 1.0–1.45 | 0.6–1.0 | 0.6–1.45 | 0.6–1.45 | ||
|
Picked particles [×103] (per micrograph) |
2387 (689) |
2791 (805) |
1641 (660) |
1618 (651) |
1491 (711) |
1489 (710) |
1480 (647) |
1772 (774) |
3168 (692) |
3429 (723) |
||
|
Final particle set [×103] (retention) (per micrograph) |
479 (20%) (138) |
793 (28%) (229) |
248 (15.1%) (100) |
278 (17.2%) (112) |
306 (21%) (146) |
337 (23%) (161) |
234 (16%) (102) |
385 (22%) (168) |
524 (17%) (115) |
733 (21%) (155) |
||
| Resolution after CTF refinement [Å] | 3.43 | 2.95 | 2.76 | 3.08 | 2.62 | 2.66 | 2.56 | 2.59 | 2.47 | 2.62 | ||
| Resolution after polishing [Å] | 3.22 | 2.80 | 2.62 | 2.87 | 2.44 | 2.39 | 2.41 | 2.37 | 2.36 | 2.37 | 2.21 | 2.39 |
| B-factor after polishing [Å2] | 188.0 (5.1) | 135.5 (6.5) | 113.6 (3.9) | 134.7 (7.4) | 91.2 (2.6) | 87.1 (2.5) | 94.3 (2.5) | 88.2 (2.8) | 87.3 (1.9) | 83.9 (2.0) | 80.5 (2.2) | 94.2 (1.9) |
| Particles to reach 3 Å after polishing [×103] | 1787 (539) | 191 (102) | 33.8 (12) | 118.7 (70) | 19.1 (5.7) | 23.6 (7.0) | 13.9 (3.8) | 13.3 (4.2) | 24.2 (5.6) | 28.5 (7.5) | 14.9 (4.4) | 33.2 (7.3) |
| Resolution from 100k particles after polishing [Å] | 3.53 (0.07) | 3.14 (0.12) | 2.77 (0.07) | 3.03 (0.12) | 2.60 (0.06) | 2.63 (0.06) | 2.56 (0.05) | 2.52 (0.06) | 2.64 (0.05) | 2.66 (0.06) | 2.51 (0.06) | 2.73 (0.05) |
| Median number of significant samples (rlnNrOfSignificantSamples) | 35 | 29 | 24 | 31 | 19 | 23 | 19 | 24 | 17 | 28 | 18 | 20 |
| Angular accuracy [deg] (rlnAccuracyRotations) | 1.09 | 1.17 | 0.96 | 1.02 | 0.88 | 0.89 | 0.83 | 0.53 | 0.95 | 0.54 | 0.85 | 0.92 |
| Translational accuracy [Å] (rlnAccuracyTranslationsAngst) | 0.58 | 0.50 | 0.38 | 0.49 | 0.36 | 0.39 | 0.32 | 0.20 | 0.39 | 0.27 | 0.34 | 0.40 |
Unless the row title indicates otherwise, the value in brackets is the estimated standard error.
The final map resolution and B-factor of each subset are highlighted in bold.