Table 1.

HPLC Conditioned for Folic Acid and Vitamin B₁₂ Analysis.

Column	Kinetex, 2.6 µm, C18, 100 Å, LC column 100 × 4.6 mm
Dimensions	100 × 4.6 mm ID
Elution type	Gradient
	Acetonitrile
Elution B:	20 mM phosphate buffer pH 3
Gradient profile	Step No	Time (min)	Sol. A	Sol. B
	0	0.5	5	95
	1	5.0	25	75
	2	5.00	5	95
	3	5.00	75	25
	4	5.00	5	95
Flow rate	1 mL/min
Col. Temp	Ambient
Detection	UV–Vis Abs.-variable wave.(UV) @ 278 & 360 nm (22 °C)
Run time	20 min
Injection volume	20µL