Table 1.

BBD for the three selected variables with coded and real values; observed results for the released protein concentration in the enzymatically treated feathers.

Trial	X1 Time (h)	X2 Feather conc. (w/v %)	X3 Keratinase (U)	X1 × X2	X2 × X3	X1 × X3	X1 × X1	X2 × X2	X3 × X3	Soluble protein conc. in FPL (µg/ml)
1	− 1(7)	− 1(1)	0(30)	1	0	0	1	1	0	797.50
2	1(21)	− 1(1)	0(30)	− 1	0	0	1	1	0	982.50
3	− 1(7)	1(3)	0(30)	− 1	0	0	1	1	0	1114.17
4	1(21)	1(3)	0(30)	1	0	0	1	1	0	1737.5
5	− 1(7)	0(2)	− 1(10)	0	0	1	1	0	1	194.16
6	1(21)	0(2)	− 1(10)	0	0	− 1	1	0	1	417.50
7	− 1(7)	0(2)	1(50)	0	0	− 1	1	0	1	402.50
8	1(21)	0(2)	1(50)	0	0	1	1	0	1	1649.1
9	0(14)	− 1(1)	− 1(10)	0	1	0	0	1	1	665.83
10	0(14)	1(3)	− 1(10)	0	− 1	0	0	1	1	1359.1
11	0(14)	− 1(1)	1(50)	0	− 1	0	0	1	1	849.16
12	0(14)	1(3)	1(50)	0	1	0	0	1	1	1774.1
13	0(14)	0(2)	0(30)	0	0	0	0	0	0	1619.1
14	0(14)	0(2)	0(30)	0	0	0	0	0	0	1670.8

conc. concentration.