Table 5.

Effect of different metal ions, detergents, surfactants, activators/inhibitors on the activity of the partially purified enzyme using the casein substrate and measured as protease.

Residual activity of protease % (Mean ± SD)
Tested	Different metal ions at conc
	1 mM	5 mM	10 mM	15 mM	20 mM
Cu2+	3.32 ± 0.50	4.66 ± 3.11	2.76 ± 1.13	2.33 ± 0.57	3.01 ± 0.33
Co2+	235.21 ± 6.31	378.61 ± 2.62	171.33 ± 2.91	151.50 ± 1.13	3.22 ± 0.01
Ca+	145.42 ± 3.01	323.79 ± 3.21	165.11 ± 6.22	136.10 ± 0.92	228.41 ± 2.52
Hg2+	18.37 ± 1.71	59.91 ± 1.82	16.00 ± 1.43	2.10 ± 0.15	2.72 ± 0.57
Mg2+	155.12 ± 0.99	281.31 ± 0.61	289.13 ± 3.95	274.40 ± 2.11	232.12 ± 1.73
Mn2+	157.81 ± 3.8	278.62 ± 0.92	98.44 ± 2.15	119.15 ± 2.32	1.23 ± 0.22
Fe2+	225.01 ± 1.34	195.71 ± 1.43	208.62 ± 1.63	411.10 ± 1.63	1577 ± 4.32
Ni2+	174.62 ± 4.02	260.22 ± 2.13	140.83 ± 1.84	134.12 ± 2.11	319.22 ± 0.05
Zn2+	244.51 ± 2.2	321.33 ± 1.24	92.66 ± 0.73	62.61 ± 1.92	0.92 ± 0.13
Control	100.00 ± 0.00	100.00 ± 0.00	100.00 ± 0.00	100.00 ± 0.00	100.0 ± 0.00

	Detergents, surfactants and solvents at conc
	0.1%	0.5%	1%	2%
Tween-20	114.62 ± 3.22	96.8 ± 1.80	127.6 ± 1.50	122.3 ± 1.1
Tween-40	113.13 ± 2.7	177 ± 3.20	164.7 ± 1.52	149.3 ± 2.0
Tween-80	96.24 ± 3.24	105 ± 0.90	122.8 ± 1.51	134.6 ± 1.4
SDS	94.54 ± 0.41	21.3 ± 1.30	35.93 ± 1.00	0.7 ± 1.10
Methanol	3.12 ± 1.04	7.0 ± 2.00	95.5 ± 0.94	100.8 ± 2.5
Ethanol	7.45 ± 2.04	5.2 ± 2.70	125.2 ± 0.51	100.9 ± 1.6
Glycerol	4.05 ± 1.03	6.6 ± 2.50	103.4 ± 1.5	104.2 ± 2.1
Triton-X100	92.23 ± 1.32	195 ± 1.10	219.6 ± 1.60	4 ± 0.00
Control	100.00 ± 0.00	100 ± 0.0	100 ± 0.00	100 ± 0.0

	Activators/ inhibitors at conc
	1 mM	5 mM	10 mM
EDTA	94.31 ± 5.52	92.33 ± 1.03	93.52 ± 0.71
DTT	102.11 ± 2.41	103.02 ± 2.05	100.23 ± 1.32
PMSF	90.71 ± 2.12	112.92 ± 2.02	120.13 ± 0.40
Control	100.00 ± 0.00	100.00 ± 0.00	100.00 ± 0.00