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. 2021 Jul 15;11:14519. doi: 10.1038/s41598-021-93654-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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JNK-pathway activity and cell polarity are not altered in the absence of MarvelD3. (A) Detection of JNK, c-Jun and their phosphorylated forms in protein extracts from 3 wild-type (+ / +) and 3 MarvelD3 knockout (−/−) pancreata by western blotting. No differences in JNK, phospho-JNK (P-JNK), c-Jun and phospho-c-Jun (P–c-Jun, Ser63 and Ser73) protein levels were observed between wild-type and knockout pancreata. Quantification of the bands (P-JNK/JNK and P–c-Jun/c-Jun) is shown on the right. Full-length blots/gels are presented in Supplementary Fig. 8. (B) Cell polarity assessment by immunolabeling of apical (ezrin, red) and basal (laminin, green) markers of epithelial (E-Cadherin, white) cells on E15.5 embryonic pancreas sections. Wild-type (+ / +) and knockout (−/−) pancreatic epithelial cells are polarized. (C) RT-qPCR evaluation of tight junction and baso-lateral marker gene expression levels in embryonic (E15.5 and E17.5) pancreata of wild-type (+ / + , green circles), heterozygous (+ /−, orange squares) and MarvelD3 knockout (−/−, red triangles) mice. Transient downregulation of claudin-3 and -7, and NaK-ATPase (Atp1a) is observed in E15.5 knockouts. (D–E) Immunolabeling of the TAMP occludin and tricellulin (green) in the wild-type and knockout E15.5 pancreatic epithelium (E-Cadherin, red). The absence of MarvelD3 does not trigger relocalization of the two other TAMPs.