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. 2021 Jul 15;4:871. doi: 10.1038/s42003-021-02401-w

Fig. 2. Functional screening methodology.

Fig. 2

a The growth phenotype was scored by comparing the mycelium growth in a given condition (i.e., agar-YNB + tested compound) vs the two following controls setting the boundaries of the growth scale: agar-YNB plate (score 0) and malt agar plate (score 4). Note that for RB5 and BB41 the agar plates contained 0.5% malt (vs 1.5% malt in positive control plate) to sustain growth. The white dotted circle indicates the fungal growth diameter (which is not always visible on pictures). b Functional phenotype scores (ranging from 0 to 4) reflect the extent of decolorization (on the industrial dyes Reactive Black 5 (RB5) and Basic Blue 41 (BB41)), browning upon phenol oxidation (on lignosulfonate (LGS)), clearing halo formation (on the soluble polyurethane Impranil® DLN (IMP)) or growth (on Avicel (AVI)). A score of 0 means no decolorization/degradation/oxidation. The pictures show examples for the different scores.