Fig. 3. Gpx4 deficiency does not affect HSC function in vivo.
a The number of HSPCs, b the relative MFI of ROS in LSK cells and LT-HSCs, and c the cell cycle of LSK cells and LT-HSCs from the pIpC-treated Gpx4flox/flox Mx-Cre mice 7 days after 5-FU treatment were measured. b The experimental schematic for serial competitive transplantation with LT-HSCs. e The chimerism of peripheral blood cells, f the chimerism of HSPCs at the 4th month after primary transplantation, g the chimerism of HSPCs at the 6th months after secondary transplantation in the recipient mice receiving LT-HSCs from the Gpx4flox/flox Vav-Cre mice. h The chimerism of peripheral blood cells, i the chimerism of HSPCs at the 7th month after primary transplantation, j the chimerism of HSPCs at the 4th month after secondary transplantation receiving LT-HSCs from the Gpx4flox/flox Mx-Cre mice. N = at least three recipient mice in each group in (e−j). Data are the mean ± SD.