Fig. 2.

Typical PLT-F scattergram of IPF. (a) Confocal laser scanning fluorescent microscopic image of isolated platelets from a healthy volunteer. The platelets were stained with a dedicated reagent of the PLT-F channel. Bar: 5 μm. (b) A typical PLT-F scattergram of whole blood from a healthy volunteer taken with an XN-1000 automated hematology analyzer. In the scattergram, platelets are plotted in a single cluster from bottom left to top right, where IPF is at the top right. Because immature platelets tend to be larger and contain higher amount of RNA than mature platelets, IPF is at the top right part of the platelet cluster colored in green. Erythrocytes are displayed as another cluster, which is located in the inset at top left. FSC: forward scattered light; SFL: side fluorescence from nucleic acid staining dye; RBC: red blood cell; PLT: platelets; IPF: immature platelet fraction.