Tween washing buffer (TWB)
| Reagent | Final concentration | Amount for 1000 μL |
|---|---|---|
| 1M Tris-HCl pH 8.0 | 5 mM | 5 μL |
| 0.5M EDTA | 5 mM | 10 μL |
| 5M NaCl | 1M | 200 μL |
| 10% Tween | 0.05% | 5 μL |
| Ultra pure water | N/A | 780 μL |
Store at 4°C, and do not store more than 2 weeks.
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