Reagent	Final concentration	Amount for 15 ul
Previously PCR-amplified DNA	N/A	5 μL
PCR Primer 1	25 μM	0,25 μL
Barcoded PCR Primer 2	25 μM	0,25 μL
100× SYBR GREEN I	1×	0,09 μL
2× PCR Master Mix	1×	5 μL
Ultra pure water	N/A	4,41 μL