| Reagent | Final concentration | Amount for 1 sample (20 ul) |
|---|---|---|
| dCTP | 10 mM | 0.375 μL |
| dGTP | 10 mM | 0.375 μL |
| dTTP | 10 mM | 0.375 μL |
| biotin-14-dATP | 0.4 mM | 9.375 μL |
| Klenow DNA polymerase I | 5 U/μL | 2.5 μL |
| NEB2 Buffer 10× | 1× | 2 μL |
| Ultra pure water | N/A | 5 μL |
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