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Methodology to study cerebral organoids.
| Microscopy | RNAseq | RTqPCR | Western blot | ELISA | |
|---|---|---|---|---|---|
| percentages | 95% | 50.5% | 34.1% | 26.2% | 7.2% |
| advantages | visualize proteins | full transcriptome | studied gene | detect/identify proteins | detect antigens |
| inconvenient | sliced in 2D | only on thousand cells located | localization lost | localization lost | localization lost |
