Figure 3 .

Systemic antisense administration improves animal behavior and muscle function. Mouse open-field behavior was assessed every 3 days for three times using locomotor activity monitors. Data obtained from each mouse/time were averaged. Representative parameters of the animal behavior are shown in (A–C), while details of all parameters are presented in Supplementary Material, Table S2. Effects of antisense therapy were further evaluated via treadmill exhaustion tests 1 week prior to the first TMX injection (baseline) and then at weeks 2 and 4 post-injection. Total running time on treadmill was recorded and expressed as the time to fatigue as the percentage of baseline time (D). Following four weekly Vivo-PMO administration, mice were put under terminal anesthesia and in situ absolute force of both TAs/mouse was measured (E). Specific muscle force is displayed as a ratio of the absolute force and the TA cross-sectional area (F). Data are shown as mean ± S.E.M, n = 4–5 mice. Statistical analysis was by one-way ANOVA (A–D) or two-way ANOVA (E, F) followed by Tukey’s multiple comparisons test, P < 0.05 (^*, ^), P < 0.01 (^**, ^^), P < 0.001 (^***), P < 0.0001 (^****).