Table 3.
Effect of solvent type and extraction time on astaxanthin content and antioxidant properties of shrimp shell extract.
Solvent type | Extraction time | Astaxanthin content | DPPHsc | FRAP |
---|---|---|---|---|
(h) | (µg/g) | (%) | (µmol Fe2+/g) | |
Petroleum ether | 2 | 10.40 m* | 23.73 h | 959.60f |
4 | 12.32 l | 26.42 g | 985.15f | |
6 | 14.24 k | 29.41f | 1038.61e | |
Hexane | 2 | 18.95 h | 18.88 k | 1122.95d |
4 | 24.32 g | 26.53 g | 1230.47c | |
6 | 31.89 d | 38.90 e | 1291.05c | |
Ethanol | 2 | 16.48 i | 19.17 k | 1058.80e |
4 | 20.86 h | 21.33 i | 1070.09e | |
6 | 26.96f | 29.67f | 1087.21e | |
Acetone | 2 | 28.16 e | 43.62 d | 1175.82d |
4 | 35.76c | 48.64c | 1263.14c | |
6 | 45.79b | 64.88b | 1424.11b | |
Petroleum ether/acetone/water (5:45:50) | 2 | 5.18p | 15.32 l | 915.65f |
4 | 6.13 op | 18.46 k | 956.04f | |
6 | 6.70o | 21.47 i | 1022.57e | |
Petroleum ether/acetone/water (15:50:35) | 2 | 5.91p | 15.00 l | 906.14f |
4 | 6.11 op | 22.56 i | 947.13f | |
6 | 9.18n | 29.62f | 1011.28e | |
Petroleum ether/acetone/water (15:75:10) | 2 | 32.14 d | 44.69 d | 1166.91d |
4 | 36.27c | 48.64c | 1295.21c | |
6 | 48.47 a | 67.64 a | 1504.90a |
*: Means within a column with the same lowercase letters are not significantly different at p < 0.05.
FRAP: Ferric Reducing Antioxidant Power
DPPHsc: scavenging activity of DPPH