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. 2021 Jul 1;24(7):102812. doi: 10.1016/j.isci.2021.102812

Figure 1.

Figure 1

Celsr3 is highly expressed in postnatal PCs

Parasagittal cerebellar sections from Celsr3-GFP mice were processed by double immunofluorescent staining with anti-Calbindin (red) and anti-GFP antibodies (green)

A-D: At postnatal day (P) 3, GFP-positive signal was identified in PCs and some fibers in deeper regions (A), with distribution similar to that of Calbindin-immunoreactivity (B), as confirmed in the merged figure (D).A few GFP-positive cells below the PC layer were negative for Calbindin (arrowheads in A, D).

E-H: At P10, GFP-immunoreactivity was visualized in cells and dendrites (E), and anti-Calbindin immunostaining showed that PC somas formed a monolayer and their dendrites invaded the molecular layer (F). Co-expression of Calbindin and Celsr3-GFP was confirmed in the merged image (H). A few GFP-positive cells were negative for Calbindin (indicated by arrowheads in E, H). I-L: At P21, co-expression of Celsr3-GFP(I) and Calbindin (J) shown in PCs (L). DAPI staining disclosed the nuclei in blue (C, G, K). Insets in D, H and L show enlarged images indicated by boxed regions respectively.