Figure 6.

Wnt5a/Celsr3/cAMP signaling is required for LTP induction

(A) Perfusion of the cAMP agonist forskolin induces EPSP typical features of LTP in both control and Celsr3 cKO groups (P> 0.05, n = 6 PCs from 3 mice in each group, Student’s t-test). Washing out of forskolin does not diminish LTP immediately.

(B) LTP cannot be induced in Fzd3 cKO PCs compared to the control (P< 0.05, n = 6 PCs from 3 mice in each group, Student’s t-test). The time of LTP induction is indicated by the arrow.

(C) Perfusion of Wnt5a induces LTP in controls, but not in Fzd3 or Celsr3 mutants (P< 0.05, n = 6 PCs from 3 mice in each group, Student’s t-test). In the control, washing out of Wnt5a does not diminish LTP immediately.

(D) Wnt5a and forskolin perfusion do not enhance the LTP induction upon single drug perfusion, and LTP induction by forskolin and Wnt5a is prevented by pre-perfusion of the cAMP antagonist-SQ22536. P< 0.05, n = 6 PCs from 3 mice in each group, Student’s t-test.

(E) The PPR is unaffected by administration of forskolin, Wnt5a or SQ22536. P> 0.05, n = 6 PCs from 3 mice in each group, Student’s t-test.