Antibodies |
|
Rabbit monoclonal anti-mGluR1 |
CST |
Cat#12551s; RRID:AB_2797953
|
Rabbit polyclonal anti-PKCα |
Sigma |
Cat#P4334; RRID:AB_477345
|
Rabbit polyclonal anti-βIII-Tubulin |
Abcam |
Cat#ab18207; RRID:AB_444319
|
Rabbit polyclonal anti-Calretinin |
Merk Millipore |
Cat#AB5054; RRID:AB_2068506
|
Mouse monoclonal anti-Parvalbumin |
Merk Millipore |
Cat#MAB1572; RRID:AB_2174013
|
Mouse polyclonal anti-Calbindin |
Sigma |
Cat#C9848; RRID:AB_476894
|
Chicken polyclonal anti-green fluorescent protein |
Abcam |
Cat#ab13970; RRID:AB_300798
|
Mouse monoclonal anti-postsynaptic density protein 95 |
Merk Millipore |
Cat# MAB1596; RRID:AB_2092365
|
Mouse monoclonal anti-synaptophysin 38 |
Abcam |
Cat#ab8049; RRID:AB_2198854
|
Rabbit polyclonal anti-mGluR1 |
Abcam |
Cat#ab27199; RRID:AB_470927
|
Guinea pig polyclonal anti-Vglut2 |
Merk Millipore |
Cat#ab2251; RRID:AB_1587626
|
|
Chemicals, peptides, and recombinant proteins |
|
Forskolin |
MCE |
Cat# HY-15371 |
SQ22536 |
MCE |
Cat# HY-100396 |
NBQX |
Sigma |
Cat# N183 |
DHPG |
MCE |
Cat# HY-12598A |
Biocytin |
Sigma |
Cat# B4261 |
Wnt5a |
R&D |
Cat#645-WN |
TTX |
Tocris |
Cat#1078 |
Streptavidin-FITC |
BD Biosciences |
Cat#434311 |
Picrotoxin |
Abcam |
Cat#ab120315 |
|
Experimental models: Organisms/strains |
|
Mouse: PCP2-Cre |
Jackson Laboratory |
JAX: 010536 |
Mouse: Celsr3-GFP |
(Ying, et al., 2009) |
NA |
Mouse: Fzd3f/f and Fzd3KI/+ |
(Qu, et al., 2014) |
NA |
Mouse: Celsr3+/− |
(Tissir, et al., 2005) |
NA |
Mouse: Celsr3f/f |
(Zhou, et al., 2008) |
NA |
|
Software and algorithms |
|
pClamp |
Molecular devices |
https://www.moleculardevices.com/applications/patch-clamp-electrophysiology#relatedproducts |
ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
Imaris |
Bitplane |
https://imaris.oxinst.cn/ |