Table 1. Examples of 12 peptides (named as in the first column) mapping on different SARS-CoV-2 proteins (column “Match”) are here reported.
Amino acids highlighted in red represent changes with respect to the translations of CDS regions reported in the reference severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome sequence (GenBank LOCUS: NC_045512.2). Pep51, pep121, and pep230 were found with a different mass spectrometry approach using the Q Exactive HF Hybrid Quadrupole-Orbitrap with an ultra-high-field analyser (Brogna, personal communication).
| Peptide
ID |
Length
(AA) |
Fragment | Match | From | To |
|---|---|---|---|---|---|
| pep51 | 27 | ESDDYI KLNGPL TVGGSC LLSGHNLAK | NSP3 | 268 | 294 |
| pep121 | 82 | L ILSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFK | 1416 | 1497 | |
| pep20 | 77 | CLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFK | 1421 | 1497 | |
| pep230 | 62 | WVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFM | NSP4 | 241 | 302 |
| pep199 | 62 | TDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQ GVNCTEVPVAIHADQLTPTWR | S | 573 | 634 |
| pep33 | 69 | DPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTW SVYSTGSNVFQTR | 578 | 646 | |
| pep190 | 74 | SVASQSIIAYTM LLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSF | 686 | 759 | |
| pep181 | 75 | SIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNR | 691 | 765 | |
| pep103 | 31 | IQDSLSSTASALGKLQDVVNQNAQALNTLVK | 934 | 964 | |
| pep25 | 77 | DCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPI CDEPTTTTSVPL | NS3 | 199 | 275 |
| pep68 | 13 | G ISP GRMAGNGGDAALALLLLDR | N | 204 | 216 |
| pep38 | 31 | EAVGTNLPLQLGFSTGVNLVAVPTGYVDTPN | NSP14 | 99 | 129 |