Table 2. Amino acid changes reported in Table 1 have been checked for their existence among the observed variations in SARS-CoV-2 sequenced isolates available GISAID at time of writing.
All of them, except one, have been already reported in humans, and only two in Italy. For each amino acid change, the number of occurrences in GISAID isolates is reported, together with details of the first human isolate recorded in GISAID with reported collection date. AA change NSP3:A274K of pep51 has never been reported in human SARS-CoV-2 sequences, but it has been found in beta-CoV genome sequences from bats (isolate hCoV-19/bat/Yunnan/RmYN01/2019, collection date 25-06-2019).
| Peptide
ID |
AA change | Observed
in human? |
#Occurrence
in human |
Observed
in Italy? |
Observed
in other than human? |
First human isolate recorded in
GISAID with reported collection date |
Collection
date |
|---|---|---|---|---|---|---|---|
| pep51 | NSP3:A274K | No | 0 | No | Yes | - | - |
| pep51 | NSP3:K280T | Yes | 2 | No | No | hCoV-19/Finland/HEL-18-471/2021 | 23/01/2021 |
| pep51 | NSP3:V286L | Yes | 1 | No | Yes | hCoV-19/USA/TX-HMH-MCoV-25096/2021 | 20/01/2021 |
| pep121 | NSP3:L1417I | Yes | 4 | No | No | hCoV-19/USA/WA-UW163/2020 | 13/03/2020 |
| pep199 | S:D614G | Yes | 728,982 | Yes | Yes | hCoV-19/Australia/NSW2153/2020 | 25/01/2020 |
| pep33 | S:R634S | Yes | 2 | No | Yes | hCoV-19/India/MH-NIV-815-3/2020 | 07/04/2020 |
| pep190 | S:S698L | Yes | 398 | Yes | No | hCoV-19/USA/AZ-TG666166/2020 | 25/03/2020 |
| pep25 | NS3:Y264C | Yes | 106 | No | No | hCoV-19/Canada/ON-S738/2020 | 09/04/2020 |
| pep68 | N:T205I | Yes | 25,665 | Yes | No | hCoV-19/Beijing/Wuhan_IME-BJ07/2020 | 29/01/2020 |
| pep68 | N:A208G | Yes | 514 | No | No | hCoV-19/USA/MD-HP00076/2020 | 11/03/2020 |