Table 2.

Phenotypic characteristics and 16S rRNA sequence alignments of colonies isolated on blood agar from oral swabs of wild-type and Amy1 knockout mice under aerobic conditions.

Colony and cell appearances	Gram stain	Catalase activity	Reference sequences	Genus	Species	GenBank accession	Percent identity
Large gray, rods	–	+	RDP	Muribacter	muris	KP278132	99.9
			HOMD	Haemophilus	haemolyticus	HM596277	93.9
Large yellow, cocci	+	+	RDP	Staphylococcus	xylosus	D83374	99.9
			HOMD	Staphylococcus	cohnii	NR_036902	98.8
Gray α-hemolytic, cocci	+	–	RDP	Streptococcus	uncultured	EU534703	99.9
			HOMD	Streptococcus	sanguinis	AF003928	96.6
Irregular large gray, cocci	+	–	RDP	Streptococcus	thoraltensis	Y09007	99.1
			HOMD	Streptococcus	salivarius	M58839	94.3
White, cocci	+	–	RDP	Streptococcus	azizii	KM609123	100
			HOMD	Streptococcus	cristatus	AB008313	95.3
White, rods	+	–	RDP	Lactobacillus	murinus	AF157049.1	99.8
			HOMD	Lactobacillus	salivarius	AF089108	94.1
Small gray, rods	+	+	RDP	Corynebacterium	Uncultured	FJ892757a	99.5
			HOMD	Corynebacterium	mastitidis	NR_026376	98.4

Cell appearance was determined under light microscopy (×100). High-quality consensus 16S rRNA sequences from colony PCR of single isolates from three mice of each genotype were aligned by the BLAST server at the Human Oral Microbiome Database (HOMD; www.homd.org/index.php) to two sets of 16S rRNA reference sequences, HOMD 16S rRNA RefSeq 15.1 (Chen et al., 2010) and sequences of the Ribosome Database Project (RDP), release 11.5 (Cole et al., 2014). The highest scoring alignments were identical between consensus sequences from wild-type and Amy1 knockout mice. Percent identities are the total nucleotide mismatches per total matching nucleotides, including gaps.