Figure 3.

Effects of genistein supplementation on the expression of the phosphorylated protein p-IRS-1 (A), p-AKT (B), GSK-3α (C), and GSK-3β (D) levels in the ovarian samples. The bar represents the standard error of the mean. Blots were repeated at least 3 times (n = 3) and a representative blot is shown. β-actin was included to ensure equal protein loading. *p < 0.05, **p < 0.01, ***p < 0.001 statistically different compared with the control group. IRS-1: insulin receptor substrate 1; AKT: protein kinase B; GSK-3α and β: glycogen synthase kinase-3 alpha and beta.