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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Nat Genet. 2021 May 13;53(6):794–800. doi: 10.1038/s41588-021-00856-5

Extended Data Fig. 4 |. Loss of PWWP reader domain function promotes DNMT3A targeting to CpG islands.

Extended Data Fig. 4 |

a) Observed DNMT3A1 wild-type and ΔPWWP enrichment at 10-kb bins overlapping CpG islands (n = 16,414) in MSCs compared to expected signal represented by enrichment at randomly-shuffled 10-kb regions (n = 16,414). b) Overlap analysis of H2AK119ub peaks, H3K27me3 peaks, and CpG islands in mouse MSCs. Jaccard index for pairwise comparisons are indicated. c) Overlap analysis of DNMT3A1 wild-type (top 5% of 10-kb bins), DNMT3A ΔPWWP (top 5% of 10-kb bins), and CpG islands in mouse MSCs. Jaccard index for pairwise comparisons are indicated. d) Enrichment heat map depicting ChIP-seq normalized reads centered at CpG islands ± 5-kb for DNMT3A1 wild-type, DNMT3A1 PWWP mutants, RING1B, H2AK119ub, H3K27me3, H3K36me2, and H3K36me3. Regions are sorted by H2AK119ub enrichment.