Skip to main content
. Author manuscript; available in PMC: 2021 Jul 16.
Published in final edited form as: Cell Rep. 2019 Jul 16;28(3):670–681.e8. doi: 10.1016/j.celrep.2019.06.014

Figure 2. The Gabra1–2 Mutation Strengthens the Interactions between the Chimeric α1–2 Subunit and CB.

Figure 2.

(A) Representative western blots showing co-immunoprecipitation levels of CB and GPN with the WT and mutant α1 subunit.

(B) Pooled quantification (n = 4 biological and technical replicates) of protein immunoprecipitation shows a significant reduction in the pull-down of the α1 subunit and significant increases in the pull-down of CB and GPN with the chimeric α1–2 subunit. Total protein expression data were normalized to GAPDH, and IP expression data were normalized to total α1 due to the decrease in total α1 expression in Gabra1–2 mutants.

Unpaired t tests with Welch’s correction were used to analyze data (*p < 0.01; **p < 0.05; #p < 0.005). Data are expressed as the percentage of WT and error bars represent mean ± SEM.