KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Ankyrin-G (staining) scaffold protein monoclonal mouse | UC Davis/NIH NeuroMab Facility | Cat# 73-146; RRID:AB_10697718 |
| Anti-GABAAR β3 mouse monoclonal | UC Davis/NIH NeuroMab Facility | Cat# 75-149; RRID: AB_2109585 |
| Anti-Cadherin, pan Rabbit polyclonal | Cell Signaling | Cat# 4068; RRID:AB_2158565 |
| Anti-Collybistin rabbit polyclonal | Synaptic Systems | Cat# 261 003; RRID:AB_2619977 |
| GABAAR α1 N-terminal rabbit polyclonal | Abcam | Cat# ab33299; RRID:AB_732498 |
| Anti-GABAAR α2 N-terminal rabbit polyclonal | Synaptic Systems | Cat# 224 103; RRID:AB_2108839 |
| GABAAR α2 C-terminal rabbit polyclonal | Phosphosolutions | No longer available |
| Anti-GABAAR γ2L mouse monoclonal | UC Davis/NIH NeuroMab Facility | Cat #75-442; RRID: AB_2617122 |
| GAPDH rabbit polyclonal | Santa Cruz | No longer available |
| Anti-Gephyrin 3B11 mouse monoclonal | Synaptic Systems | Cat# 147 111; RRID:AB_887719 |
| Anti-Gephyrin mAb7a mouse monoclonal | Synaptic Systems | Cat# 147 011; RRID:AB_887717 |
| Anti-Gephyrin RbmAb7a rabbit monoclonal | Synaptic Systems | Cat# 147 008; RRID:AB_2619834 |
| Anti-GFP Antibody, Unconjugated chicken polyclonal | Cell Signaling | Cat# 2555; RRID:AB_10692764 |
| Anti-Sodium Channel, Pan antibody mouse monoclonal | Sigma Aldrich | Cat# S8809; RRID:AB_477552 |
| Anti-Synapsin 1 antibody mouse monoclonal | Synaptic Systems | Cat# 106 011; RRID:AB_2619772 |
| Anti-VGAT antibody guinea pig polyclonal | Synaptic Systems | 131 004; RRID:AB_887873 |
| Donkey anti-mouse peroxidase conjugated | Jackson ImmunoResearch | Cat# 715-036-151; RRID:AB_2340774 |
| Donkey anti-rabbit peroxidase conjugated | Jackson ImmunoResearch | Cat# 711-036-152; RRID:AB_2340590 |
| Goat anti-guinea pig Alexa Fluor 647 | ThermoFisher Scientific | Cat# A-21450; RRID:AB_2735091 |
| Goat anti-mouse Alexa Fluor 488 | ThermoFisher Scientific | Cat# A28175; RRID:AB_2536161 |
| Goat anti-rabbit Alexa Fluor 568 | ThermoFisher Scientific | Cat# A-11011; RRID:AB_143157 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 1 M CaCl2 | Sigma Aldrich | Cat#: 21114 |
| 1 M KCl | Sigma Aldrich | Cat#: 60121 |
| 1 M MgCl2 | Sigma Aldrich | Cat#: 63020 |
| 10x Phosphate Buffered Saline (PBS) | ThermoFisher Scientific | Cat#: BP399-4 |
| 32% PFA stock solution | Electron Miscroscopy Sciences | Cat#: 15714S |
| Acetic acid, glacial | ThermoFisher Scientific | Cat#: A38-212 |
| Bovine Serum Albumin (BSA) | Sigma Aldrich | Cat#: A7906 |
| Cresyl violet acetate | Sigma Aldrich | Cat#: C-5042 |
| CsCl | Sigma Aldrich | Cat#: C4036 |
| CsOH | Sigma Aldrich | Cat#: C8518 |
| D-Glucose | Sigma Aldrich | Cat#: G8270 |
| Dehydrated milk | Amazon | https://amazon.com/Emergency-Essentials-Instant-Nonfat-Milk/dp/B003SQ98EC |
| Ethanol, 200 proof | VWR | Cat#: 89125-172 |
| Ethylene glycol-bis(2-aminoethylether)- N,N,N′,N′-tetraacetic acid (EGTA) | Sigma Aldrich | Cat#: E3889 |
| Ethylenediaminetetraacetic acid (EDTA) | Sigma Aldrich | Cat#: E9884 |
| EZ-Link Sulfo-NHS-SS-Biotin | ThermoFisher Scientific | Cat#: 21328T |
| Fisher Healthcare Tissue-Plus O.C.T. Compound | ThermoFisher Scientific | Cat#: 23-730-571 |
| GABA | Sigma Aldrich | Cat#: A2129 |
| GIBCO B27 (50x) | ThermoFisher Scientific | Cat#: 17504044 |
| GIBCO GlutaMAX Supplement | ThermoFisher Scientific | Cat#: 35050061 |
| GIBCO Penicillin/streptomycin (10,000 U/mL) | ThermoFisher Scientific | Cat#: 15140122 |
| GIBCO Trypsin (2.5%), no phenol red | ThermoFisher Scientific | Cat#: 15090046 |
| Glycine | VWR | Cat#: 0167 |
| Invitrogen 1 M HEPES buffer solution | ThermoFisher Scientific | Cat#: 15630080 |
| Invitrogen HBSS, 10X, no phenol red, no calcium, no magnesium | ThermoFisher Scientific | Cat#: 14185-052 |
| Invitrogen MES SDS Running Buffer 20x | Thermofisher Scientific | Cat#: NP0002 |
| Invitrogen NuPAGE LDS Sample Buffer 4x | ThermoFisher Scientific | Cat#: NP0007 |
| Invitrogen ProLong Gold Antifade Mounting Reagent | Thermo Fisher Scientific | Cat#: P36930 |
| Invitrogen Protein A Dynabeads | ThermoFisher Scientific | Cat#: 10001D |
| Isofluorane | Piramal Healthcare | Cat#: NDC 66794-013-25 |
| Kainic Acid (Kainate) | Sigma Aldrich | Cat#: K0250 |
| Kynurenic Acid | Sigma Aldrich | Cat#: K337 |
| L-glutamine | Sigma Aldrich | Cat#: G8540 |
| Laminin | Sigma Aldrich | Cat#: L2020 |
| Mannitol | Sigma Aldrich | Cat#: M4125 |
| Meglumine (NMDG) | Sigma Aldrich | Cat#: M9179 |
| Methanol | Sigma Aldrich | Cat#: 179337 |
| Mg-ATP | Sigma Aldrich | Cat#: A9187 |
| MgSO4 | Sigma Aldrich | Cat#: M3409 |
| Na-ascorbate | Sigma Aldrich | Cat#: A7631 |
| Na-GTP | Sigma Aldrich | Cat#: G8877 |
| NaCl | Sigma Aldrich | Cat#: S7653 |
| NaF | Sigma Aldrich | Cat#: S7920 |
| NaH2PO4 | Sigma Aldrich | Cat#: S9638 |
| NaHCO3 | Sigma Aldrich | Cat#: S6297 |
| Neurobasal-A Medium, serum-free | ThermoFisher Scientific | Cat#: 10888022 |
| Normal Goat Serum (NGS) | Abcam | Cat#: ab7481 |
| Pierce Streptavidin Agarose beads | ThermoFisher Scientific | Cat#: 20347 |
| Pierce BCA Protein Assay Kit | ThermoFisher Scientific | Cat#: 23225 |
| Pierce ECL Western Blotting Substrate | ThermoFisher Scientific | Cat#: 32106 |
| Poly-L-Lysine hydrobromide | Sigma Aldrich | Cat#: P1274 |
| Roche cOmplete, Mini Protease Inhibitor Cocktail | Sigma Aldrich | Cat#: 11836153001 |
| Roche PhosSTOP | Sigma Aldrich | Cat#: 4906845001 |
| Sodium orthovanadate | Sigma Aldrich | Cat#: 450243 |
| Sodium pyruvate | Sigma Aldrich | Cat#: P3662 |
| Sucrose | VWR | Cat#: 0335 |
| Tetrodotoxin (TTX) | Tocris | Cat#: 1069 |
| Thiourea | Sigma Aldrich | Cat#: 8658 |
| Tris | VWR | Cat#: 0497 |
| Triton X-100 | Sigma Aldrich | Cat#: T8787 |
| Tween-20 | Sigma Aldrich | Cat#: P2287 |
| β-mercaptoethanol | Sigma Aldrich | Cat#: 63689 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Gabra1-2(−/−) Mouse: Gabra1-2(+/−) Mouse: Gabra1-2(+/+) | This paper | N/A |
| Mouse: pHlourin-α2(+/+) Mouse: pHlourin-α2(+/+)/Gabra1-2(+/+) | Nakamura et al., 2016 | N/A |
| Mouse: Gabra2-1(+/−)/Gabra1-2(+/−) | Hines et al., 2018; This paper | N/A |
| Oligonucleotides | ||
| Gabra1-2 Genotyping Forward Primer: 5′-GGGTC GACAACTATTTCACCAAGAGAGG-3′ | Integrated DNA Technologies | N/A |
| Gabra1-2 Genotyping Reverse Primer: 5′-GGGC GGCCGCTTATCGGTCGATTTTGCTGACGC-3′ | Integrated DNA Technologies | N/A |
| Gabra1-2 Sequencing Primer 1: 5′-TGCCAGGGA GTCTAACCGT-3′ | Integrated DNA Technologies | N/A |
| Gabra1-2 Sequencing Primer 2: 5′-CAAATAGCA GCGGAAAGGCT-3′ | Integrated DNA Technologies | N/A |
| Gabra1-2 Sequencing Primer 3: 5′-CTGCCAGG GAGTCTAACCGT-3′ | Integrated DNA Technologies | N/A |
| Gabra1-2 Sequencing Primer 4: 5′-GACAGTCG GTCGATTTTGCTG-3′ | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| GraphPad Prism, v.7.01 | GraphPad Software | RRID:SCR_002798 https://www.graphpad.com |
| Image Lab, v.5.0 | Bio-Rad | RRID:SCR_014210 http://www.bio-rad.com |
| ImageJ v.2.0.0 | Fiji | RRID:SCR_002285 https://imagej.net |
| LabChart v.7.3.8 | AD Instruments | RRID:SCR_001620 https://www.adinstruments.com/products/labchart |
| MetaMorph, v.7.8.0.0 | Molecular Devices | RRID:SCR_002368 https://www.moleculardevices.com |
| Mini-Analysis Software v.5.6.4 | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis/ |
| MotorMonitor | Kinder Scientific | http://kinderscientific.com/products/open_field |
| NIS-Elements, v.4.20 | Nikon | RRID:SCR_014329 https://www.microscope.healthcare.nikon.com/products/software |
| pClamp v.10.3 | Moleclular Devices | RRID:SCR_011323 https://www.moleculardevices.com |
| Sirenia Acquisition, v.1.7.10 | Pinnacle Technology | RRID:SCR_016183 https://www.pinnaclet.com |
| Other | ||
| Axon Axopatch 200B Microelectrode Amplifier | Molecular Devices | Cat#: 200B https://www.moleculardevices.com/sites/default/files/en/assets/data-sheets/dd/cns/axon-axopatch-200b-microelectrode-amplifier.pdf |
| ChemiDoc XRS | Bio-Rad | Cat#: 1708265 http://www.bio-rad.com/en-uk/product/chemidoc-xrs-system?ID=NINJHRKG4 |
| Crytostat | ThermoFisher Scientific | Cat#: HM525 No longer available |
| Leica VT1000 S Vibrating blade microtome | Leica | Cat#: VT1000 S https://www.leicabiosystems.com/histology-equipment/sliding-and-vibrating-blade-microtomes/vibrating-blade-microtome/products/leica-vt1000-s/ |
| Nikon A1 confocal scanning laser microscope | Nikon | Cat#: A1HD25 https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/a1hd25-a1rhd25 |
| Nikon Eclipse E800 Brightfield Microscope | Nikon | No longer available |