Skip to main content
. Author manuscript; available in PMC: 2021 Jul 16.
Published in final edited form as: Biochim Biophys Acta Bioenerg. 2018 Nov 6;1860(1):22–29. doi: 10.1016/j.bbabio.2018.11.001

Figure 4. Cholesterol does not affect mVDAC1 voltage gating.

Figure 4.

Normalized conductance (left panels), open probability (middle panels), and gating parameters (right panels) obtained for mVDAC1 WT (A) and E73Q mutant (B) on multichannel membranes formed of PLE in the absence and presence of 25% (w/w) of cholesterol in 1 M KCl. In all panels, the membrane-bathing solutions were buffered with 5 mM HEPES at pH 7.4. Data are means of 3–9 experiments ± SD. Solid lines in middle panels (in A: light red for PLE and grey for PLE with cholesterol; in B: light grey for PLE and light green for PLE with cholesterol) are the fits of Popen plots with the Boltzmann equation (eq. 2) and fitting parameters n and V0 are shown in right panels. Right panels: There is no significant difference between n and V0 gating parameters (p>0.05, t-test with unequal variance and two-tailed distribution).