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. 2021 Jul 2;9:628569. doi: 10.3389/fbioe.2021.628569

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Copyright © 2021 Ren, Liu, Wei, Dai and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirm of pSET152-Pj23119-AsCHS-PermE-RgTAL-PkasO-Sc4CL plasmids. (A) Schematic diagram of heterologous genes expression cassette (Pj23119-AsCHS-PermE-RgTAL-PkasO-Sc4CL) in pSET152. (B) qRT-PCR analysis expression of AsCHS, RgTAL, and Sc4CL in AbΔery:CT4 with AbΔery as control. (C) qRT-PCR analysis expression of AsCHS, RgTAL, and Sc4CL in WT:CT4 with WT as control. Transcript levels were obtained individually after drawing a standard curve using Ct value and copy number logarithm as the horizontal and vertical coordinates with a serial copy number gradient plasmid as a control. Error bars indicate the standard deviations from three independent replicates.