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. 2021 Jul 16;19:112. doi: 10.1186/s12958-021-00788-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Day 4 pregnancy in Tsg101^d/d mice. (A) Mice (10–11-week-old) received 2.5 IU of eCG and hCG and were bred with stud male mice. On day 4 at 11 AM, the uteri were collected and one horn was flushed with warm M16 media. A set of representative images of the retrieved embryos are shown. See Table 3. (B) Histological analysis of day 4 pregnant uterine sections. Unflushed uterine horns were used for this experiment. Paraffin-embedded sections were stained with hematoxylin and eosin. lm, longitudinal muscle; cm, circular muscle; s, stroma; le, luminal epithelium. Areas demarcated with a black rectangle are magnified in the right panels. Sections from two different Tsg101^d/d uteri are shown as #1 and #2. Black arrowheads indicate the luminal epithelia. Two sections from different subjects were chosen and heights of the luminal epithelia were measured in several different areas. The measurement of epithelial heights is shown in graph. Bars represent means ± SEM. (C) Immunofluorescence staining of E-cadherin (epithelial cell marker) and desmin (stromal cell marker) was performed in one set of day 4 pregnant mice to show cell identity. Unflushed uterine horns were used for this experiment. Areas demarcated with a white square are magnified in the lower panels. DNA was counterstained with TO-PRO™-3-Iodide (1:250)