Fig. 2.

Experimental protocol to assess the effects of AAT-Fc following elastase instillation (A). Mice received AAT-Fc, pAAT, IgG1-Fc or vehicle 21 days after PPE instillation and the effects in the lungs were analyzed 32 days after PPE (AAT Fc PPE day 32, pAAT PPE day 32, IgG1-Fc PPE day 32, and PBS PPE day 32, respectively). A control group received saline instillation and lungs were analyzed 32 days later. Separate groups of mice were analyzed 21 days after saline or PPE instillation without any treatment (saline day 21 and PPE day 21, respectively). Cst values (B), lung tissue sections (C), MLI (D), and alveolar surface area (S) (E) values were compared. n = 8 in each group. *p < 0.05 by Tukey–Kramer test