Table 2.
Comparison of DNA-based NGS, RNA-based NGS, IHC and FISH results among 14 lung cancer cases
| Case | Sex | Age | Diagnosis | DNA-based NGS | RNA-based NGS | IHC | FISH |
|---|---|---|---|---|---|---|---|
| 1 | M | 54 | ADC |
EML4-intergenic (intron13: intergenic) WDR43-ALK (3′ intron1: 3′ intron19) |
EML4-ALK (exon13: exon20) |
ALK+ | N/A |
| 2 | F | 32 | ADC |
intergenic-EML4 (intergenic: intron6) ALS2CR11-ALK (3′ intron9: 3’intron19) ALK-RBKS (intron19: intron5) |
EML4-ALK (exon6: exon20) |
ALK+ | + |
| 3 | M | 45 | ADC |
EML4-intergenic (intron6: intergenic) PLXNA4-ALK (3′ intron3: 3′ intron19) intergenic-EML4 (intergenic: intron6) |
EML4-ALK (exon6: exon20) |
ALK+ | + |
| 4 | M | 28 | ADC |
EML4-ALK (intron13: intron3) ALK-ALK (intron3ins9: intron19) GALM-EML4 (intron4: intron13) ALK-intergenic (intron19: intergenic) |
EML4-ALK (exon13: exon20) |
ALK+ | + |
| 5 | M | 63 | ADC |
EML4-ALK (5′ intron6: 5′ intron19) ALK-ALK (3′ intron18: 3′ intron19) IL1RAPL2-ALK (5′ intron6: 5′ intron18) ALK-PTCHD1 (3′ intron18: 3′ intron1) |
EML4-ALK (exon6: exon20) |
ALK+ | + |
| 6 | F | 54 | ADC |
EML4-ALK (intron6ins39: intron4) ALK-ALK (intron4ins57: intron19) |
N/A | N/A | N/A |
| 7 | F | 54 | ADC |
EML4-EML4 (5′ intron6: 5′ intron6) EML4-ALK (3′ intron6: 3′ intron19) |
EML4-ALK (exon6: exon20) EML4-ALK (exon6ins33: exon20) |
ALK+ | + |
| 8 | M | 50 | ADC |
EML4-EML4 (5′ intron6: 5′ intron6) EML4-ALK (3′ intron6ins4: 3′ intron19) |
EML4-ALK (exon6: exon20) |
ALK+ | + |
| 9 | M | 75 | ADC |
EML4-LCLAT1 (5′ intron13: 5′ intron1) LCLAT1-ALK (3′ intron1: 3′ intron19) |
EML4-ALK (exon13: exon20) |
ALK+ | − |
| 10 | M | 59 | ADC |
EML4-NEB (intron13: intron24) NEB-ALK (intron24: intron19) |
EML4-ALK (exon13: exon20) |
ALK+ | + |
| 11 | F | 44 | ADC |
EML4-KCNQ3 (intron6: intron1) KCNQ3-ALK (intron1: intron19) ALK-EML4 (intron19: intron6) |
EML4-ALK (exon6: exon20) |
ALK+ | + |
| 12 | F | 43 | ADC |
EML4-RUNX1 (intron5: intron5) RUNX1-ALK (intron6: intron19) |
EML4-ALK (exon5: exon20) RUNX1-ALK (exon6: exon20) |
ALK+ | − |
| 13 | M | 56 | ADC |
EML4-ZNF362 (intron13: intron1) ZNF362-ALK (intron1: intron19) |
EML4-ALK (exon13: exon20) |
ALK+ | N/A |
| 14 | M | 31 | ADC |
EML4-RPIA (intron13: intron3) MAP4K3-ALK (intron2: intron19) |
EML4-ALK (exon13: exon20) |
ALK+ | + |
F: female; M: male; ADC: adenocarcinoma; N/A: not available; +: positive results; −: negative results